Li Xiao-yan, Yu Ni, Jiang Li-hua, Wei Bing, Ding Shu-zhen

Nursing Department, First Affiliated Hospital of Dalian Medical University, Dalian  116011, Liaoning Province, China

Li Xiao-yan, Nurse-in-charge, Nursing Department, First Affiliated Hospital of Dalian Medical University, Dalian  116011, Liaoning Province, China
lixiaoyan_1982@126.com

Correspondence to: Ding Shu-zhen,Nurse-incharge, Nursing Department, First Affiliated Hospital of Dalian Medical University, Dalian  116011, Liaoning Province, China
DSZ51@163.com
Abstract
BACKGROUND: Plenty of studies have shown that 3M multi-enzyme cleaner to clean implantation instruments of orthopedics manually is better than that without multi-enzyme cleaner, but there is no widely accepted measures to judge success of cleaning implantation instruments of orthopedics. Endotoxin detection benefits establishing evaluation criteria of cleaning implantation instruments of orthopedics successfully, and reflecting the cleaning effect to a certain extent in order to effectively apply the multi-enzyme cleaner.
OBJECTIVE: To detect endotoxin quantitatively in the surface of implantation instruments of orthopedics and the cleaner after using 3M multi-enzyme cleaner to clean implantation instruments of orthopedics manually, so as to evaluate the cleaning effect of implantation instruments of orthopedics.
METHODS: A total of 600 implantation instruments of orthopedics were selected randomly and cleaned by 3M multi-enzyme cleaner. The samples of the surface of instruments (before and after clean medical instruments) and in multi-enzyme cleaner were collected. Using Ltoy Lalt method, 8 EU/mL endotoxin was obtained and diluted to eight different concentrations. The reaction time was measured, standard curve equation was established, and the endotoxin in the sample was quantified according to the equation.
RESULTS AND CONCLUSION: In the maximum cleaning effectiveness of the multi-enzyme cleaner, the endotoxin content in the multi-enzyme cleaner was less than 6.0 EU/mL after cleaning. Results show that endotoxin quantification in the multi-enzyme cleaner can be used to judge success of cleaning implantation instruments of orthopedics.

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